Creation of Resistant Genotypes Against Fungal Diseases in Wheat Using the CRISPR-Cas 9 Method (A Review)
Wheat is one of the main food crops cultivated in the world. Although wheat yield has increased significantly in recent years, wheat production is predicted to face great difficulties due to global climate change and diseases. Pests and diseases cause 20-40% of global food losses and cause 220 billion US dollars in agricultural trade losses every year. Diseases such as wheat rusts, consisting of leaf, stem and stripe rusts, fusarium wilt, etc., are significant problems for farmers worldwide and cause major yield losses in one of the most important staple crops in the world. Currently, targeted genome editing is a rapid technology for generating mutations in candidate genes. CRISPR/Cas9-based genome editing can be effectively used to target genes involved in resistance to biotic and abiotic stress factors. In the research, the pleiotropic effect and susceptibility mechanism of candidate genes in wheat plant resistant samples to various fungal diseases were comparatively studied using CRISPR/Cas9 technology and the advantages and disadvantages of each were identified.