Advances in Biology & Earth Sciences

Using cicer extract, selenium nanoparticles (SeNPs) were produced in this study. The synthesis was completed quickly and affordably without the use of any hazardous materials. To describe SeNPs, FTIR Spectroscopy, Zetapotantials and SEM were used. The FTIR spectrum analysis of the generated selenium nanoparticles shows that the phytochemicals in the cicer extract act as reductants during the SeNP manufacturing stage. Phytochemicals from cicer extract operate as reductants during the SeNP synthesis phase, according to spectrum analysis.  The zeta potential of the produced SeNP nanoparticles was found to be -24.67 mV. SeNPs' minimum inhibitory concentrations (MIC) for Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231 were determined to be 3.125, 1.56 and 0.39 µg/mL, respectively. The osteosarcoma U2OS and the human breast cancer MCF-7 cell lines were used to investigate SeNPs' anticancer capabilities using the MTT test. During the first 24 hours, MCF-7 cells exhibited significant suppression at a concentration of 50 µg/mL of SeNPs, whereas U2OS cancer cell lines showed negligible inhibition. The degree of suppression in both cell lines decreases with dose and time.