Advances in Biology & Earth Sciences

The light desynchronization was realized by putting the male Wistar rats under the effects of uninterrupted light illumination for 14 days. The levels of collapsin-response mediator protein 2 (CRMP2) were measured with indirect ELISA test in the hypothalamus and epiphysis of the rats. The CRMP2 levels were significantly higher in the experimental animals relative to the controls. Then the animals were culled into two groups: 1) control and 2) experimental. The rats of both groups were subjected to a light desynchronization and prior to and after it they were tested in the elevated plus-maze. The control animals were administered the inactive CRMP2 24 h prior to desynchronization and a week later since its beginning, the experimental animals were administered active CRMP2 in the same schedule. The number of hangings did not change in the experimental rats, while in the controls their values significantly declined. The total duration of grooming in the closed arms increased drastically in the control animals, whereas in the experimental rats it decreased. The maze center escaping latency increased significantly in the experimental animals. The cortisol levels in the serum of the experimental animals were significantly lower relative to the controls. The results indicate to involvement of CRMP2 in molecular regulation of circadian rhythm.